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Urine DNA Storage Tube

The discovery of circulating cell-free DNA (cfDNA) in peripheral blood has been extensively

studied and documented. The detected cfDNA can originate from a tumor, fetus or transplanted

organs. More recently, it has been determined that small amounts of cfDNA can pass from the

blood through the kidney barrier into urine. The obvious advantage of urine sampling makes urine

a truly useful source of fetal and tumor cfDNA, a clear advantage over peripheral blood. The cfDNA

isolated from urine sample can be used for various applications, including disease monitoring

(diagnosis, prognosis and treatment efficacy) and as a screening tool for hereditary conditions.

Urine DNA Storage kit are designed for the rapid preservation of nucleic acids from fresh

urine specimens. The Urine Storage Tube is a plastic, closed, evacuated tube that contains

x ml of Urine Preservative additive. The Urine Preservative is a general purpose reagent that

contains urine enzyme inhibitors and a cell preservative in a liquid medium. It prevents the growth

of Gram-negative and Gram-positive bacteria and fungi, stabilizes nucleated cells, preventing the

release of cellular genomic DNA, and inhibits nuclease mediated degradation of circulating

cell-free DNA (cfDNA) in urine samples during storage and transportation for up to 7 days

at 6 °C to 37 °C. It also inactivates viruses, allowing the resulting non-infectious samples

to be handled and shipped safely.

Instructions for use

1. Wash hands with soap and water prior to sample collection.
2. Twist the cap off the Urine Collection Cup in a counterclockwise direction (Do not reveal the protective cover),

and invert the cap on a clean table, taking care not to touch the inside of the cap with your fingers.

3. Collect urine in the specimen collection cup up to volumes 30-80 ml (about 60 ml). Note: Collect

midstream urine. First flow urine is not recommended for collection.
4. Screw the cap tightly back onto the cup and mix by gentle inversion 3 to 5 times.
5. Remove the protective cover and reveal the collection hole on the top of the cap.

6. Turn Urine DNA Storage Tube upside down and insert into the collection hole, insure the urine

sample is fully sucked into the storage tube (about 10ml for each tube).

Note: It is recommended that the urine specimen is transferred into 2 storage tubes within 2 hours of collection.

7. Remove the storage tube from the collection hole and mix by gentle inversion 8-10 times.
8. After collection, transport and store preserved urine samples within the recommended temperature

range (6 °C to 37 °C).

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cfDNA Extraction

1. For isolation of cell-free DNA, centrifuge specimens at room temperature at 4000 rpm for 10 minutes,

or follow extraction kit manufacturer’s instructions.
2. Carefully transfer the supernatant to a new tube using a pipette without disturbing the pellet.

Order Information:

Code number	Description
CW2657S	Urine cfDNA Storage Container, Individually packed, Sterile